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Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time

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dc.contributor.author Henriques, R
dc.contributor.author Lelek, M
dc.contributor.author Fornasiero, EF
dc.contributor.author Valtorta, F
dc.contributor.author Zimmer, C
dc.contributor.author Mhlanga, Musa
dc.date.accessioned 2010-08-30T15:27:37Z
dc.date.available 2010-08-30T15:27:37Z
dc.date.issued 2010-08-31
dc.identifier.citation Henriques, R, Lelek, M, Fornasiero, EF. 2010. Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time. CSIR 3rd Biennial Conference: Science Real and Relevant, CSIR International Convention Centre, Pretoria, South Africa, 30 August – 1 September 2010, pp 7 en
dc.identifier.uri http://hdl.handle.net/10204/4221
dc.description CSIR 3rd Biennial Conference: Science Real and Relevant, CSIR International Convention Centre, Pretoria, South Africa, 30 August – 1 September 2010 en
dc.description.abstract Super-resolution microscopy using photoswitchable molecules requires fast and efficient software for single molecule detection, localization, drift correction and visualization. However, there is still a dearth of freely available, flexible and easy-to-use reconstruction software coupled with image acquisition. Here, we present QuickPALM, an Image plugin, enabling real-time reconstruction of 3D super-resolution images during acquisition and drift correction. We illustrate its application by reconstructing Cy5-labelled microtubules with a resolution of 40-50 nm en
dc.language.iso en en
dc.publisher CSIR en
dc.subject Synthetic biology en
dc.subject Super resolution images en
dc.subject QuickPALM en
dc.subject 3D Images en
dc.title Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time en
dc.type Conference Presentation en
dc.identifier.apacitation Henriques, R., Lelek, M., Fornasiero, E., Valtorta, F., Zimmer, C., & Mhlanga, M. (2010). Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time. CSIR. http://hdl.handle.net/10204/4221 en_ZA
dc.identifier.chicagocitation Henriques, R, M Lelek, EF Fornasiero, F Valtorta, C Zimmer, and Musa Mhlanga. "Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time." (2010): http://hdl.handle.net/10204/4221 en_ZA
dc.identifier.vancouvercitation Henriques R, Lelek M, Fornasiero E, Valtorta F, Zimmer C, Mhlanga M, Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time; CSIR; 2010. http://hdl.handle.net/10204/4221 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Henriques, R AU - Lelek, M AU - Fornasiero, EF AU - Valtorta, F AU - Zimmer, C AU - Mhlanga, Musa AB - Super-resolution microscopy using photoswitchable molecules requires fast and efficient software for single molecule detection, localization, drift correction and visualization. However, there is still a dearth of freely available, flexible and easy-to-use reconstruction software coupled with image acquisition. Here, we present QuickPALM, an Image plugin, enabling real-time reconstruction of 3D super-resolution images during acquisition and drift correction. We illustrate its application by reconstructing Cy5-labelled microtubules with a resolution of 40-50 nm DA - 2010-08-31 DB - ResearchSpace DP - CSIR KW - Synthetic biology KW - Super resolution images KW - QuickPALM KW - 3D Images LK - https://researchspace.csir.co.za PY - 2010 T1 - Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time TI - Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time UR - http://hdl.handle.net/10204/4221 ER - en_ZA


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