Super-resolution microscopy using photoswitchable molecules requires fast and efficient software for single molecule detection, localization, drift correction and visualization. However, there is still a dearth of freely available, flexible and easy-to-use reconstruction software coupled with image acquisition. Here, we present QuickPALM, an Image plugin, enabling real-time reconstruction of 3D super-resolution images during acquisition and drift correction. We illustrate its application by reconstructing Cy5-labelled microtubules with a resolution of 40-50 nm
Reference:
Henriques, R, Lelek, M, Fornasiero, EF. 2010. Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time. CSIR 3rd Biennial Conference: Science Real and Relevant, CSIR International Convention Centre, Pretoria, South Africa, 30 August – 1 September 2010, pp 7
Henriques, R., Lelek, M., Fornasiero, E., Valtorta, F., Zimmer, C., & Mhlanga, M. (2010). Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time. CSIR. http://hdl.handle.net/10204/4221
Henriques, R, M Lelek, EF Fornasiero, F Valtorta, C Zimmer, and Musa Mhlanga. "Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time." (2010): http://hdl.handle.net/10204/4221
Henriques R, Lelek M, Fornasiero E, Valtorta F, Zimmer C, Mhlanga M, Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time; CSIR; 2010. http://hdl.handle.net/10204/4221 .
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