Browsing Journal Articles by Subject "Biocatalysis"

Now showing items 1-15 of 15

Advances in enzyme immobilisation

Author: Brady, D ; Jordaan, J Date: 10 Jul 2009 Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit ... Read more
Bacterial nitrilases and their regulation

Author: Chhiba, Varsha P ; Van der Westhuyzen, Christiaan W ; Bode, M ; Brady, D Date: Jun 2019 Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important ... Read more
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Decision making in the development of a biocatalytic route for resolution of S-naproxen: from screening to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Jun 2010 The non-steroidal anti-inflammatory drug naproxen is most effective as the single S-naproxen enantiomer. However, typical synthetic routes to naproxen yield the racemate of both the R and the S stereoisomers. Biocatalysts can be used to resolve ... Read more
Diamination by n-coupling using a commercial laccase

Author: Wellington, Kevin W ; Steenkamp, P ; Brady, D Date: Feb 2010 Nuclear diamination of p-hydrobenzoquinones with aromatic and aliphatic primary amines was catalyzed by a immobilised commercial laccase, Denilite II Base, from Novozymes. The amine and the p-hydrobenzoquinone was reacted under mild conditions ... Read more
Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous

Author: Chhiba, Varsha P ; Mathiba, Kgama ; Van der Westhuyzen, Christiaan W ; Steenkamp, Paul A ; Rashamuse, JK ; Stoychev, Stoyan H ; Bode, ML ; Brady, D Date: Dec 2018 Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase ... Read more
Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Kwezi, Lusisizwe ; Brady, D Date: Apr 2012 A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed ... Read more
Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSynTM polymer microspheres

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Mar 2012 The use of enzymes in industrial applications is limited by their instability, cost and difficulty in their recovery and re-use. Immobilisation is a technique which has been shown to alleviate these limitations in biocatalysis. Here we describe ... Read more
An integrated chemo-enzymatic route for preparation of ß-thymidine, a key intermediate in the preparation of antiretrovirals

Author: Gordon, GER ; Bode, ML ; Visser, Daniel F ; Lepuru, MJ ; Zeevaart, JG ; Ragubeer, N ; Ratsaka, M ; Walwyn, DR ; Brady, D Date: Jan 2011 A chemo-enzymatic method for production of ß-thymidine, an intermediate in the synthesis of antiretrovirals, is described. Guanosine and thymine were converted by means of enzymatic transglycosylation to yield 5-methyluridine (5-MU), which ... Read more
A Laccase-catalysed one-pot synthesis of aminonaphthoquinones and their anticancer activity

Author: Wellington, Kevin W ; Kolesnikova, NI Date: May 2012 Nuclear monoamination of a 1,4-naphthohydroquinone with primary aromatic amines was catalysed by the commercial laccase, Novozym 51003, from Novozymes to afford aminonaphthoquinones. The synthesis was accomplished by reacting a mixture of the ... Read more
A one-pot laccase-catalysed synthesis of 5,6-dihydroxylated benzo[b]furans and catechol derivatives, and their anticancer activity

Author: Wellington, Kevin W ; Qwebani-Ogunleye, T ; Kolesnikova, NI ; Brady, D ; De Koning, CB Date: Apr 2013 A commercial laccase, Suberase® from Novozymes, was used to catalyse the synthesis of 5,6-dihydroxylated benzo[b]furans and catechol derivatives. The yields were, in some cases, similar to or better than that obtained by other enzymatic, ... Read more
A one-pot laccase-catalysed synthesis of coumestan derivatives and their anticancer activity

Author: Qwebani-Ogunleye, Tozama ; Kolesnikova, Natalia I ; Steenkamp, Paul A ; De Koning, CB ; Brady, D ; Wellington, Kevin W Date: Dec 2016 Suberase®, a commercial laccase from Novozymes, was used to catalyse the synthesis of coumestans. The yields, in some cases, were similar to or better than that obtained by other enzymatic, chemical or electrochemical syntheses. The compounds ... Read more
Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

Author: Visser, Daniel F ; Hennessy F ; Rashamuse J ; Pletschke B ; Brady D Date: Aug 2010 Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ... Read more
Substrate profiling of the cobalt nitrile hydratase from Rhodococcus rhodochrous ATCC BAA 870

Author: Mashweu, AR ; Bode, ML ; Brady, D ; Chhiba, Varsha P Date: 2020 The aromatic substrate profile of the cobalt nitrile hydratase from Rhodococcus rhodochrous ATCC BAA 870 was evaluated against a wide range of nitrile containing compounds (>60). To determine the substrate limits of this enzyme, compounds ... Read more

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Browsing Journal Articles by Subject "Biocatalysis"

Browsing Journal Articles by Subject "Biocatalysis"

Browse

All of ResearchSpace

This Collection

Quick Links

Legislation and compliance

General Enquiries

Social Connect