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Browsing by Author "Crampton, Michael C"

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Analyzing the molecular mechanism of lipoprotein localization in Brucella

Author: Goolab, Shivani ; Roth, Robyn L ; Crampton, Michael C ; Van Heerden, H Date: Oct 2015 Bacterial lipoproteins possess diverse structure and functionality, ranging from bacterial physiology to pathogenic processes. As such many lipoproteins, originating from Brucella are exploited as potential vaccines to countermeasure brucellosis ... Read more
Cattle tick vaccine researchers join forces in CATVAC

Author: Schetters, T ; Bishop, R ; Crampton, Michael C ; Kopácek, P ; Lew-Tabor, A Date: Feb 2016 A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14–15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC). Read more c b
Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197

Author: Roth, Robyn L ; Van Zyl, Petrus J ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mamputha, Sipho ; Buthelezi, Sindisiwe G ; Crampton, Michael C Date: Apr 2017 The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application ... Read more
Development of a flagellin surface display expression system in a moderate thermophile, Bacillus halodurans Alk36

Author: Crampton, Michael C ; Berger, E ; Reid, S ; Louw, M Date: Jun 2007 This study relates to the development of an alkaliphilic, thermotolerant, Gram-positive isolate, Bacillus halodurans Alk36, for the over-production and surface display of chimeric gene products. This bacterium continuously over-produces ... Read more
Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

Author: Berger, E ; Crampton, Michael C ; Nxumalo, NP ; Louw, ME Date: Aug 2011 Through modification of the flagellin type III secretion pathway of Bacillus halodurans heterologous peptides could be secreted into the medium as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced ... Read more
Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression

Author: Berger, E ; Du Plessis, E ; Gerber, I ; Crampton, Michael C ; Nxumalo, NP ; Louw, M Date: Jan 2009 The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as ... Read more
Serum antibody immunoreactivity and safety of native porcine and recombinant zona pellucida vaccines formulated with a non-Freund’s adjuvant in horses

Author: Nolan, M ; Schulman, M ; Botha, A ; Human, A ; Roth, Robyn L ; Crampton, Michael C ; Bertschinger, H Date: Feb 2019 Commercial and regulatory limitations associated with native porcine zona pellucida (pZP) vaccines formulated with Freund’s adjuvants may be overcome by developing effective recombinant ZP vaccines (reZP) and identifying alternative adjuvant ... Read more
To 'display' or not to 'display'- that is the peptide

Author: Crampton, Michael C ; Berger, E ; Louw, ME Date: Nov 2008 Microbial cell surface display is the anchoring of a heterologous protein or peptide (passenger) to the outside of the cell wall as a fusion to a cell surface associated protein (carrier). This technology has been used extensively for both ... Read more
Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans

Author: Crampton, Michael C Date: Jul 2010 This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation ... Read more

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Browsing by Author "Crampton, Michael C"

Browsing by Author "Crampton, Michael C"

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