Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression
Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression
The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases
Reference:
Berger, E., Du Plessis, E., Gerber, I., et al. 2009. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. Applied and Environmental Microbiology, Vol. 75(1), pp 1-23
Berger, E., Du Plessis, E., Gerber, I., Crampton, M. C., Nxumalo, N., & Louw, M. (2009). Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. http://hdl.handle.net/10204/3314
Berger, E, E Du Plessis, I Gerber, Michael C Crampton, NP Nxumalo, and M Louw "Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression." (2009) http://hdl.handle.net/10204/3314
Berger E, Du Plessis E, Gerber I, Crampton MC, Nxumalo N, Louw M. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. 2009; http://hdl.handle.net/10204/3314.
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