dc.contributor.author |
Bain, RES
|
|
dc.contributor.author |
Woodall, C
|
|
dc.contributor.author |
Elliott, J
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|
dc.contributor.author |
Arnold, BF
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|
dc.contributor.author |
Tung, R
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|
dc.contributor.author |
Morley, R
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|
dc.contributor.author |
du Preez, M
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|
dc.contributor.author |
Bartram, JK
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|
dc.contributor.author |
Davis, AP
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|
dc.contributor.author |
Gundry, SW
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|
dc.contributor.author |
Pedley, S
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|
dc.date.accessioned |
2016-05-16T10:21:47Z |
|
dc.date.available |
2016-05-16T10:21:47Z |
|
dc.date.issued |
2015-10 |
|
dc.identifier.citation |
Bain, RES, Woodall, C, Elliott, J, Arnold, BF, Tung, R, Morley, R, du Preez, M, Bartram, JK, Davis, AP, Gundry, SW and Pedley, S. 2015. Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters. PLoS One, pp 1-13 |
en_US |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.uri |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140997
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/8546
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|
dc.description |
Copyright: 2015 PLoS One |
en_US |
dc.description.abstract |
The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in lowincome countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti- Tray1. AT was compared with IDEXX Colilert-181 and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-181 for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
PLOS ONE |
en_US |
dc.relation.ispartofseries |
Workflow;15926 |
|
dc.subject |
Escherichia coli |
en_US |
dc.subject |
Filtration |
en_US |
dc.subject |
Sewage effluent |
en_US |
dc.subject |
Sub-tropical study |
en_US |
dc.subject |
Surface water |
en_US |
dc.subject |
Water Quality |
en_US |
dc.title |
Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Bain, R., Woodall, C., Elliott, J., Arnold, B., Tung, R., Morley, R., ... Pedley, S. (2015). Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters. http://hdl.handle.net/10204/8546 |
en_ZA |
dc.identifier.chicagocitation |
Bain, RES, C Woodall, J Elliott, BF Arnold, R Tung, R Morley, M du Preez, et al "Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters." (2015) http://hdl.handle.net/10204/8546 |
en_ZA |
dc.identifier.vancouvercitation |
Bain R, Woodall C, Elliott J, Arnold B, Tung R, Morley R, et al. Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters. 2015; http://hdl.handle.net/10204/8546. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Bain, RES
AU - Woodall, C
AU - Elliott, J
AU - Arnold, BF
AU - Tung, R
AU - Morley, R
AU - du Preez, M
AU - Bartram, JK
AU - Davis, AP
AU - Gundry, SW
AU - Pedley, S
AB - The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in lowincome countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti- Tray1. AT was compared with IDEXX Colilert-181 and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-181 for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.
DA - 2015-10
DB - ResearchSpace
DP - CSIR
KW - Escherichia coli
KW - Filtration
KW - Sewage effluent
KW - Sub-tropical study
KW - Surface water
KW - Water Quality
LK - https://researchspace.csir.co.za
PY - 2015
SM - 1932-6203
T1 - Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters
TI - Evaluation of an inexpensive growth medium for direct detection of Escherichia coli in temperate and sub-tropical waters
UR - http://hdl.handle.net/10204/8546
ER -
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en_ZA |