dc.contributor.author |
Mthunzi-Kufa, Patience
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|
dc.contributor.author |
Lee, WM
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|
dc.contributor.author |
Riches, AC
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dc.contributor.author |
Brown, CTA
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dc.contributor.author |
Gunn-Moore, FJ
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dc.contributor.author |
Dholakia, K
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dc.date.accessioned |
2011-12-14T08:45:07Z |
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dc.date.available |
2011-12-14T08:45:07Z |
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dc.date.issued |
2010-05 |
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dc.identifier.citation |
Mthunzi, P, Lee, WM, Riches, AC et al. 2010. Intracellular dielectric tagging for improved optical manipulation of mammalian cells. IEEE Journal of Selected Topics in Quantum Electronics, Vol 16(3), pp 608-618 |
en_US |
dc.identifier.issn |
1077-260X |
|
dc.identifier.uri |
http://ieeexplore.ieee.org/Xplore/login.jsp?reload=true&url=http%3A%2F%2Fieeexplore.ieee.org%2Fiel5%2F2944%2F5477232%2F05361341.pdf%3Farnumber%3D5361341&authDecision=-203
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|
dc.identifier.uri |
http://hdl.handle.net/10204/5410
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|
dc.description |
Copyright: 2010 IEEE. This is an ABSTRACT ONLY |
en_US |
dc.description.abstract |
Optical micromanipulation of transparent microparticles such as cellularmaterials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cellmanipulation and sorting, highlighting some of the key experiments over the last twenty years.We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellularmaterial and may enable new sorting techniques within microfluidic systems. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
IEEE |
en_US |
dc.relation.ispartofseries |
Workflow request;6085 |
|
dc.subject |
Intracellular dielectric tagging |
en_US |
dc.subject |
Optical cell sorting |
en_US |
dc.subject |
Axial forces |
en_US |
dc.subject |
Scattering forces |
en_US |
dc.subject |
Quantum electronics |
en_US |
dc.subject |
Mammalian cells |
en_US |
dc.title |
Intracellular dielectric tagging for improved optical manipulation of mammalian cells |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Mthunzi, P., Lee, W., Riches, A., Brown, C., Gunn-Moore, F., & Dholakia, K. (2010). Intracellular dielectric tagging for improved optical manipulation of mammalian cells. http://hdl.handle.net/10204/5410 |
en_ZA |
dc.identifier.chicagocitation |
Mthunzi, P, WM Lee, AC Riches, CTA Brown, FJ Gunn-Moore, and K Dholakia "Intracellular dielectric tagging for improved optical manipulation of mammalian cells." (2010) http://hdl.handle.net/10204/5410 |
en_ZA |
dc.identifier.vancouvercitation |
Mthunzi P, Lee W, Riches A, Brown C, Gunn-Moore F, Dholakia K. Intracellular dielectric tagging for improved optical manipulation of mammalian cells. 2010; http://hdl.handle.net/10204/5410. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Mthunzi, P
AU - Lee, WM
AU - Riches, AC
AU - Brown, CTA
AU - Gunn-Moore, FJ
AU - Dholakia, K
AB - Optical micromanipulation of transparent microparticles such as cellularmaterials relies upon the application of optical forces that are crucially dependent on the refractive index contrast between the particle and the surrounding medium. We briefly review the application of optical forces for cellmanipulation and sorting, highlighting some of the key experiments over the last twenty years.We then introduce a new technique for enhancing the dielectric contrast of mammalian cells, which is a result of cells naturally taking up microspheres from their environment. We explore how these intracellular dielectric tags can influence the scattering and gradient forces upon these cells from an externally applied optical field. We show that intracellular polymer microspheres can serve as highly directional optical scatterers and that scattering forces can enable sorting through axial guiding onto laminin-coated glass coverslips upon which the selected cells adhere. Such internal dielectric tagging presents a simple, inexpensive, sterile technique to enhance optical manipulation procedures for cellularmaterial and may enable new sorting techniques within microfluidic systems.
DA - 2010-05
DB - ResearchSpace
DP - CSIR
KW - Intracellular dielectric tagging
KW - Optical cell sorting
KW - Axial forces
KW - Scattering forces
KW - Quantum electronics
KW - Mammalian cells
LK - https://researchspace.csir.co.za
PY - 2010
SM - 1077-260X
T1 - Intracellular dielectric tagging for improved optical manipulation of mammalian cells
TI - Intracellular dielectric tagging for improved optical manipulation of mammalian cells
UR - http://hdl.handle.net/10204/5410
ER - |
en_ZA |