dc.contributor.author |
Mthunzi-Kufa, Patience
|
|
dc.contributor.author |
Dholakia, K
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|
dc.contributor.author |
Gunn-Moore, F
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|
dc.date.accessioned |
2011-09-30T09:55:20Z |
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dc.date.available |
2011-09-30T09:55:20Z |
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dc.date.issued |
2010-07 |
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dc.identifier.citation |
Mthunzi, P, Dholakia, K and Gunn-Moore, F. 2010. Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation. Journal of Biomedical Optics, Vol 15(4), pp 041507 |
en_US |
dc.identifier.issn |
1083-3668 |
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dc.identifier.uri |
http://hdl.handle.net/10204/5189
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|
dc.description |
Copright: 2010 Society of Photo-optical Instrumentation Engineers (SPIE) |
en_US |
dc.description.abstract |
Recently, femtosecond laser pulses have been utilized for the targeted introduction of genetic matter into mammalian cells. This rapidly expanding and developing novel optical technique using a tightly focused laser light beam is called phototransfection. Extending previous studies, we show that femtosecond lasers can be used to phototransfect a range of different cell lines, and specifically that this novel technology can also transfect mouse embryonic stem cell colonies with 25% efficiency. Notably, we show the ability of differentiating these cells into the extraembryonic endoderm using phototransfection. Furthermore, we present two new findings aimed at optimizing the phototransfection method and improving applicability: first, the influence of the cell passage number on the transfection efficiency is explored and, second, the ability to enhance the transfection efficiency via whole culture treatments. Our results should encourage wider uptake of this methodology |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Society of Photo-optical Instrumentation Engineers (SPIE) |
en_US |
dc.relation.ispartofseries |
Workflow request;6084 |
|
dc.subject |
Phototransfection |
en_US |
dc.subject |
Femtosecond |
en_US |
dc.subject |
Laser pulses |
en_US |
dc.subject |
Cell arresting |
en_US |
dc.subject |
Neuroblastomas |
en_US |
dc.subject |
Pluripotent stem cells |
en_US |
dc.subject |
Embryonic stem cell differentiation |
en_US |
dc.subject |
Biomedical optics |
en_US |
dc.subject |
Mammalian cells |
en_US |
dc.title |
Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Mthunzi, P., Dholakia, K., & Gunn-Moore, F. (2010). Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation. http://hdl.handle.net/10204/5189 |
en_ZA |
dc.identifier.chicagocitation |
Mthunzi, P, K Dholakia, and F Gunn-Moore "Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation." (2010) http://hdl.handle.net/10204/5189 |
en_ZA |
dc.identifier.vancouvercitation |
Mthunzi P, Dholakia K, Gunn-Moore F. Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation. 2010; http://hdl.handle.net/10204/5189. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Mthunzi, P
AU - Dholakia, K
AU - Gunn-Moore, F
AB - Recently, femtosecond laser pulses have been utilized for the targeted introduction of genetic matter into mammalian cells. This rapidly expanding and developing novel optical technique using a tightly focused laser light beam is called phototransfection. Extending previous studies, we show that femtosecond lasers can be used to phototransfect a range of different cell lines, and specifically that this novel technology can also transfect mouse embryonic stem cell colonies with 25% efficiency. Notably, we show the ability of differentiating these cells into the extraembryonic endoderm using phototransfection. Furthermore, we present two new findings aimed at optimizing the phototransfection method and improving applicability: first, the influence of the cell passage number on the transfection efficiency is explored and, second, the ability to enhance the transfection efficiency via whole culture treatments. Our results should encourage wider uptake of this methodology
DA - 2010-07
DB - ResearchSpace
DP - CSIR
KW - Phototransfection
KW - Femtosecond
KW - Laser pulses
KW - Cell arresting
KW - Neuroblastomas
KW - Pluripotent stem cells
KW - Embryonic stem cell differentiation
KW - Biomedical optics
KW - Mammalian cells
LK - https://researchspace.csir.co.za
PY - 2010
SM - 1083-3668
T1 - Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation
TI - Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation
UR - http://hdl.handle.net/10204/5189
ER - |
en_ZA |