dc.contributor.author |
Du Preez, M
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dc.contributor.author |
Van der Merwe, Martina R
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dc.contributor.author |
Cumbana, A
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dc.contributor.author |
Le Roux, Wouter J
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dc.date.accessioned |
2011-02-01T06:40:29Z |
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dc.date.available |
2011-02-01T06:40:29Z |
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dc.date.issued |
2010-10 |
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dc.identifier.citation |
Du Preez, M, Van der Merwe, MR, Cumbana, A and Le Roux, WJ. 2010. A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique. Water SA, Vol. 36(5), pp 615-620 |
en_US |
dc.identifier.issn |
0378-4738 |
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dc.identifier.uri |
http://www.wrc.org.za/Pages/DisplayItem.aspx?ItemID=8837&FromURL=/Pages/KH_AnnualReports.aspx%3F
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dc.identifier.uri |
http://hdl.handle.net/10204/4803
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dc.description |
Copyright: 2010 Water Research Commission |
en_US |
dc.description.abstract |
This study determined whether the estuarine and freshwater environment in Beira, Mozambique, serves as a reservoir of Vibrio cholerae O1 and O139. Ninety-nine estuarine water samples were collected at 6 sites in Beira. An additional 54 samples were collected from rural areas around Beira which included 3 freshwater lake samples, 15 river, 5 pond, and 4 estuarine water samples, and an equivalent number of sediment samples, collected from the same sites as the water samples. In addition, fish scales from 5 ocean fish and 1 deep sea water sample were also collected. The samples were analysed for the presence of V. cholerae O1 and O139 using culture methods, the direct fluorescent antibody (DFA) method and polymerase chain reaction (PCR) using a single-primer pair for the ompW gene and a semi-nested PCR selecting for the ctxA gene, encoding subunit A of cholera toxin. DFA results showed 37 V. cholerae O1- and 6 O139-positive samples. Vibrio cholerae O1 and O139 were observed on the scales of 4 of the 5 fish. The findings of the study provided in situ evidence for V. cholerae O1 and O139, predominantly as viable but non-culturable cells in the aquatic environment of Beira. This is the first record of the presence of V. cholerae O139 in the estuarine environment on the coast of Africa. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Water Research Commission |
en_US |
dc.relation.ispartofseries |
Journal Article |
|
dc.subject |
Health |
en_US |
dc.subject |
Water quality |
en_US |
dc.subject |
Ecosystem |
en_US |
dc.subject |
Biomonitoring |
en_US |
dc.subject |
Vibrio cholerae O1 |
en_US |
dc.subject |
Vibrio cholerae O139 |
en_US |
dc.subject |
Estuaries |
en_US |
dc.subject |
Sediments |
en_US |
dc.title |
A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Du Preez, M., Van der Merwe, M. R., Cumbana, A., & Le Roux, W. J. (2010). A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique. http://hdl.handle.net/10204/4803 |
en_ZA |
dc.identifier.chicagocitation |
Du Preez, M, Martina R Van der Merwe, A Cumbana, and Wouter J Le Roux "A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique." (2010) http://hdl.handle.net/10204/4803 |
en_ZA |
dc.identifier.vancouvercitation |
Du Preez M, Van der Merwe MR, Cumbana A, Le Roux WJ. A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique. 2010; http://hdl.handle.net/10204/4803. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Du Preez, M
AU - Van der Merwe, Martina R
AU - Cumbana, A
AU - Le Roux, Wouter J
AB - This study determined whether the estuarine and freshwater environment in Beira, Mozambique, serves as a reservoir of Vibrio cholerae O1 and O139. Ninety-nine estuarine water samples were collected at 6 sites in Beira. An additional 54 samples were collected from rural areas around Beira which included 3 freshwater lake samples, 15 river, 5 pond, and 4 estuarine water samples, and an equivalent number of sediment samples, collected from the same sites as the water samples. In addition, fish scales from 5 ocean fish and 1 deep sea water sample were also collected. The samples were analysed for the presence of V. cholerae O1 and O139 using culture methods, the direct fluorescent antibody (DFA) method and polymerase chain reaction (PCR) using a single-primer pair for the ompW gene and a semi-nested PCR selecting for the ctxA gene, encoding subunit A of cholera toxin. DFA results showed 37 V. cholerae O1- and 6 O139-positive samples. Vibrio cholerae O1 and O139 were observed on the scales of 4 of the 5 fish. The findings of the study provided in situ evidence for V. cholerae O1 and O139, predominantly as viable but non-culturable cells in the aquatic environment of Beira. This is the first record of the presence of V. cholerae O139 in the estuarine environment on the coast of Africa.
DA - 2010-10
DB - ResearchSpace
DP - CSIR
KW - Health
KW - Water quality
KW - Ecosystem
KW - Biomonitoring
KW - Vibrio cholerae O1
KW - Vibrio cholerae O139
KW - Estuaries
KW - Sediments
LK - https://researchspace.csir.co.za
PY - 2010
SM - 0378-4738
T1 - A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique
TI - A survey of Vibrio cholerae O1 and O139 in estuarine waters and sediments of Beira, Mozambique
UR - http://hdl.handle.net/10204/4803
ER -
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en_ZA |