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Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans

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dc.contributor.author Crampton, Michael C
dc.date.accessioned 2010-09-28T11:38:57Z
dc.date.available 2010-09-28T11:38:57Z
dc.date.issued 2010-07
dc.identifier.citation Crampton, M.C. 2010. Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1, 2010, pp 18 en
dc.identifier.uri http://hdl.handle.net/10204/4381
dc.description 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010 en
dc.description.abstract This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB. en
dc.language.iso en en
dc.publisher CSIR en
dc.subject Heterologous genes en
dc.subject Bacillus halodurans en
dc.subject Genetics en
dc.subject Microorganisms en
dc.subject Industrial microorganisms en
dc.subject Genetics of Industrial Microorganisms (GIM 2010) en
dc.title Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans en
dc.type Conference Presentation en
dc.identifier.apacitation Crampton, M. C. (2010). Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. CSIR. http://hdl.handle.net/10204/4381 en_ZA
dc.identifier.chicagocitation Crampton, Michael C. "Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans." (2010): http://hdl.handle.net/10204/4381 en_ZA
dc.identifier.vancouvercitation Crampton MC, Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans; CSIR; 2010. http://hdl.handle.net/10204/4381 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Crampton, Michael C AB - This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB. DA - 2010-07 DB - ResearchSpace DP - CSIR KW - Heterologous genes KW - Bacillus halodurans KW - Genetics KW - Microorganisms KW - Industrial microorganisms KW - Genetics of Industrial Microorganisms (GIM 2010) LK - https://researchspace.csir.co.za PY - 2010 T1 - Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans TI - Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans UR - http://hdl.handle.net/10204/4381 ER - en_ZA


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