Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation
Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation
Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from four South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA isolated; this DNA was sequenced and analysed using BLAST
Reference:
Visser, DF, Hennessy, F, Chhiba, VP et al. 2006. Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. University of the Free State, pp 1
Visser, D. F., Hennessy, F., Chhiba, V. P., Lakay, F., van Heerden, E., Piater, L., ... Brady, D. (2006). Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. http://hdl.handle.net/10204/2877
Visser, Daniel F, F Hennessy, Varsha P Chhiba, F Lakay, E van Heerden, L Piater, C Moller, D Litthauer, and D Brady. "Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation." (2006): http://hdl.handle.net/10204/2877
Visser DF, Hennessy F, Chhiba VP, Lakay F, van Heerden E, Piater L, et al, Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation; 2006. http://hdl.handle.net/10204/2877 .
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