The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec (-) strain as well as in a B. subtilis sacU (HY) strain.
Reference:
Louw, M, et al. 1994. Cloning and sequencing the degs-degu operon from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 42(1), pp 78-84
Louw, M., Reid, S., James, D., & Watson, T. (1994). Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis. http://hdl.handle.net/10204/2022
Louw, M, SJ Reid, DM James, and TG Watson "Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis." (1994) http://hdl.handle.net/10204/2022
Louw M, Reid S, James D, Watson T. Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis. 1994; http://hdl.handle.net/10204/2022.
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