dc.contributor.author |
Mugwanda, Kanganwiro
|
|
dc.contributor.author |
Hamese, Saltiel
|
|
dc.contributor.author |
Van Zyl, Winschau F
|
|
dc.contributor.author |
Prinsloo, E
|
|
dc.contributor.author |
Du Plessis, M
|
|
dc.contributor.author |
Dicks, L
|
|
dc.contributor.author |
Thimiri Govindaraj, Deepak B
|
|
dc.date.accessioned |
2023-03-17T08:37:34Z |
|
dc.date.available |
2023-03-17T08:37:34Z |
|
dc.date.issued |
2023-01 |
|
dc.identifier.citation |
Mugwanda, K., Hamese, S., Van Zyl, W.F., Prinsloo, E., Du Plessis, M., Dicks, L. & Thimiri Govindaraj, D.B. 2023. Recent advances in genetic tools for engineering probiotic lactic acid bacteria. <i>Bioscience Reports, 43(1).</i> http://hdl.handle.net/10204/12678 |
en_ZA |
dc.identifier.issn |
1573-4935 |
|
dc.identifier.issn |
0144-8463 |
|
dc.identifier.uri |
DOI: 10.1042/BSR20211299
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/12678
|
|
dc.description.abstract |
Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One of the emerging applications of synthetic biology is to exploit the link between microorganisms, biologics, and human health. To exploit this link, it is critical to select effective synthetic biology tools for use in appropriate microorganisms that would address unmet needs in human health through the development of new game-changing applications and by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered appropriate chassis organisms that can be genetically engineered for therapeutic and industrial applications. Here, we have reviewed comprehensively various synthetic biology techniques for engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, and recombineering. In addition, we also discussed heterologous protein expression systems used in engineering probiotic LAB. By combining computational biology with genetic engineering, there is a lot of potential to develop next-generation synthetic LAB with capabilities to address bottlenecks in industrial scale-up and complex biologics production. Recently, we started working on Lactochassis project where we aim to develop next generation synthetic LAB for biomedical application. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://pubmed.ncbi.nlm.nih.gov/36597861/ |
en_US |
dc.source |
Bioscience Reports, 43(1) |
en_US |
dc.subject |
Clustered regularly interspaced short palindromic repeats |
en_US |
dc.subject |
CRISPR |
en_US |
dc.subject |
Homologous recombination |
en_US |
dc.subject |
Lactic acid bacteria |
en_US |
dc.subject |
Probiotic engineering |
en_US |
dc.subject |
Synthetic biology |
en_US |
dc.title |
Recent advances in genetic tools for engineering probiotic lactic acid bacteria |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
27 |
en_US |
dc.description.note |
2023 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
en_US |
dc.description.cluster |
Next Generation Health |
en_US |
dc.description.impactarea |
Synthetic Nanobiotech Biomachs |
en_US |
dc.identifier.apacitation |
Mugwanda, K., Hamese, S., Van Zyl, W. F., Prinsloo, E., Du Plessis, M., Dicks, L., & Thimiri Govindaraj, D. B. (2023). Recent advances in genetic tools for engineering probiotic lactic acid bacteria. <i>Bioscience Reports, 43(1)</i>, http://hdl.handle.net/10204/12678 |
en_ZA |
dc.identifier.chicagocitation |
Mugwanda, Kanganwiro, Saltiel Hamese, Winschau F Van Zyl, E Prinsloo, M Du Plessis, L Dicks, and Deepak B Thimiri Govindaraj "Recent advances in genetic tools for engineering probiotic lactic acid bacteria." <i>Bioscience Reports, 43(1)</i> (2023) http://hdl.handle.net/10204/12678 |
en_ZA |
dc.identifier.vancouvercitation |
Mugwanda K, Hamese S, Van Zyl WF, Prinsloo E, Du Plessis M, Dicks L, et al. Recent advances in genetic tools for engineering probiotic lactic acid bacteria. Bioscience Reports, 43(1). 2023; http://hdl.handle.net/10204/12678. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Mugwanda, Kanganwiro
AU - Hamese, Saltiel
AU - Van Zyl, Winschau F
AU - Prinsloo, E
AU - Du Plessis, M
AU - Dicks, L
AU - Thimiri Govindaraj, Deepak B
AB - Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One of the emerging applications of synthetic biology is to exploit the link between microorganisms, biologics, and human health. To exploit this link, it is critical to select effective synthetic biology tools for use in appropriate microorganisms that would address unmet needs in human health through the development of new game-changing applications and by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered appropriate chassis organisms that can be genetically engineered for therapeutic and industrial applications. Here, we have reviewed comprehensively various synthetic biology techniques for engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, and recombineering. In addition, we also discussed heterologous protein expression systems used in engineering probiotic LAB. By combining computational biology with genetic engineering, there is a lot of potential to develop next-generation synthetic LAB with capabilities to address bottlenecks in industrial scale-up and complex biologics production. Recently, we started working on Lactochassis project where we aim to develop next generation synthetic LAB for biomedical application.
DA - 2023-01
DB - ResearchSpace
DP - CSIR
J1 - Bioscience Reports, 43(1)
KW - Clustered regularly interspaced short palindromic repeats
KW - CRISPR
KW - Homologous recombination
KW - Lactic acid bacteria
KW - Probiotic engineering
KW - Synthetic biology
LK - https://researchspace.csir.co.za
PY - 2023
SM - 1573-4935
SM - 0144-8463
T1 - Recent advances in genetic tools for engineering probiotic lactic acid bacteria
TI - Recent advances in genetic tools for engineering probiotic lactic acid bacteria
UR - http://hdl.handle.net/10204/12678
ER -
|
en_ZA |
dc.identifier.worklist |
26475 |
en_US |