Author:Visser, Daniel F; Hennessy F; Rashamuse J; Pletschke B; Brady DDate:Aug 2010Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ...Read more